Guilty Gear: Dust Strikers, is a continuation of the Guilty Gears universe, with all 21 of the previous version's fighters, each with their own special moves and classic special moves such as Psych Burst, Dust Attack and Roman Cancel among others.

The gameplay is reminiscent of Super Smash Bros., with vertical scrolling and various item power-ups being thrown randomly onto the screen during a match. There are six game modes to compete in such as story mode, arcade, one-on-one and the ability to play against four players wirelessly using Wi-Fi in a "battle royals rules" match. Each level will feature traps such as fire and bomb traps among others.

Players can also customize the Robo-Ky fighter using a factory, with the ability to unlock special moves by competing in minigames such as arm wrestling, billiards and sword master, where the player uses the stylus to slice up falling bales of hay before hitting the ground.